Testing for IBD

CALPROTECTIN: ELISA TEST

In the laboratory, the faecal sample is prepared and put onto a sample plate.

An ELISA test uses antibodies to detect a specific protein in a liquid sample. It also quantifies the amount of that protein in the sample. This can be used for diagnosis, monitoring and testing.

Alpha Laboratories supplies kits that contain all the reagents required to carry out an ELISA assay for testing of Calprotectin.

It also provides laboratories with an automated machine which will run the ELISA test and read the results. This reads the intensity of colour in the test, enabling the laboratory to

quantify the amount of that protein in the sample (Quantification). The higher the amount of calprotectin, the brighter the intensity of the colour.